Biocompatible Condensation Reaction for the Labeling of Terminal Cysteine Residues on Proteins

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Biocompatible Condensation Reaction for the Labeling of Terminal Cysteine Residues on Proteins

Ren, Hongjun; Xiao, Fei; Zhan, Ke; Kim, Young-Pil; Xie, Hexin; Xia, Zuyong; Rao, Jianghong

Angewandte Chemie, December 14, 2009, Volume: 48, Issue: 51

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Going live: A protein‐labeling method based on the use of a single amino acid tag—an N‐terminal cysteine residue—and small‐molecule probes containing a cyanobenzothiazole (CBT) unit has been used for the specific fluorescence labeling of proteins in vitro and at the surface of live cells (see scheme). This simple ligation reaction proceeds with a high degree of specificity under physiological conditions. Rd: a rhodamine dye; TEV: tobacco etch virus.

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