Expression and purification of the recombinant mustard trypsin inhibitor (MTI2) in Escherichia coli

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Expression and purification of the recombinant mustard trypsin inhibitor (MTI2) in Escherichia coli

Stefan, A.(University of Bologna (Italy)); Ugolini, L; Martelli, E; Palmieri, S; Hochkoeppler, A

Journal of bioscience and bioengineering, 20/Oct , Volume: 108, Issue: 4

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The mustard trypsin inhibitor 2, MTI2, was expressed in Escherichia coli. A specific procedure for its production and purification is described. The recombinant protein was recovered by protein extraction from the insoluble fraction, then renatured and purified by ion exchange and gel filtration chromatography. Finally, the inhibitory activity against trypsin was also determined.

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Stefan, A.(University of Bologna (Italy)) | Ugolini, L | Martelli, E | Palmieri, S | Hochkoeppler, A

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