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Chang, Hui-Bo; Zou, Ji-Zhen; He, Cai; Zeng, Rui; Li, Yuan-Yuan; Ma, Fei-Fei; Liu, Zhuo; Ye, Hui; Wu, Jian-Xin
Chinese medical journal, 11/2015, Volume: 128, Issue: 22Journal Article
Background: DNA hypomethylation of long interspersed nuclear elements- 1 (LINEs- 1 ) occurs during carcinogenesis, whereas intbmaation addressing LINE-1 methylation in Wilms tumor (WT) is limited. The main purpose of our study was to quantity, LINE-1 methylation levels and evaluate their relationship with relative telomere length (TL) in WT. Methods: We investigated LINE-1 methylation and relative TL using bisulfite-polymerase chain reaction (PCR) pyrosequencing and quantitative PCR, respectively, in 20 WT tissues, 10 normal kidney tissues and a WT cell line. Significant changes were analyzed by t-tests. Results: LINE-1 methylation levels were significantly lower (P 〈 0.05) and relative TLs were sigmificantly shorter (P 〈 0.05) in WT compared with normal kidney. There was a significant positive relationship between LINE- 1 methylation and relative TL in WT (r = 0.671, P = 0.001 ). LINE- 1 Methylation levels were significantly associated with global DNA methylation (r = 0.332, P 〈 0.01 ). In addition, relative TL was shortened and LINE- 1 methylation was decreased in a WT cell line treated with the hypomethylating agent 5-aza-2'-deoxycytidine compared with untreated WT cell line. Conclusion: These results suggest that LINE-1 hypomethylation is common and may be linked to telomere shortening in WT.
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