E-resources
PDF-
Bal, Antonin; Brengel-Pesce, Karen; Gaymard, Alexandre; Quéromès, Grégory; Guibert, Nicolas; Frobert, Emilie; Bouscambert, Maude; Trabaud, Mary-Anne; Allantaz-Frager, Florence; Oriol, Guy; Cheynet, Valérie; d’Aubarede, Constance; Massardier-Pilonchery, Amélie; Buisson, Marlyse; Lupo, Julien; Pozzetto, Bruno; Poignard, Pascal; Lina, Bruno; Fassier, Jean-Baptiste; Morfin, Florence; Trouillet-Assant, Sophie; Adnot, Jerôme; Alfaiate, Dulce; Bergeret, Alain; Boibieux, André; Bonnet, Florent; Brunel-Dalmas, Florence; Caire, Eurydice; Charbotel, Barbara; Chiarello, Pierre; Cotte, Laurent; Durupt, François; Escuret, Vanessa; Fascia, Pascal; Fontaine, Juliette; Gaillot-Durand, Lucie; Gillet, Myriam; Godinot, Matthieu; Gueyffier, François; Josset, Laurence; Lahousse, Matthieu; Lozano, Hélène; Makhloufi, Djamila; Milon, Marie-Paule; Moll, Frédéric; Narbey, David; Nazare, Julie-Anne; Oria, Fatima; Perry, Marielle; Pitiot, Virginie; Prudent, Mélanie; Rabilloud, Muriel; Samperiz, Audrey; Schlienger, Isabelle; Simon, Chantal; Valette, Martine
Scientific reports, 07/2021, Volume: 11, Issue: 1Journal Article
A comprehensive clinical and microbiological assessments of COVID-19 in front-line healthcare workers (HCWs) is needed. Between April 10th and May 28th, 2020, 319 HCWs with acute illness were reviewed. In addition to SARS-CoV-2 RT-PCR screening, a multiplex molecular panel was used for testing other respiratory pathogens. For SARS-CoV-2 positive HCWs, the normalized viral load, viral culture, and virus neutralization assays were performed weekly. For SARS-CoV-2 negative HCWs, SARS-CoV-2 serological testing was performed one month after inclusion. Among the 319 HCWs included, 67 (21.0%) were tested positive for SARS-CoV-2; 65/67 (97.0%) developed mild form of COVID-19. Other respiratory pathogens were found in 6/66 (9.1%) SARS-CoV-2 positive and 47/241 (19.5%) SARS-Cov-2 negative HCWs (p = 0.07). The proportion of HCWs with a viral load > 5.0 log10 cp/mL (Ct value < 25) was less than 15% at 8 days after symptom onset; 12% of HCWs were positive after 40 days (Ct > 37). More than 90% of cultivable virus had a viral load > 4.5 log10 cp/mL (Ct < 26) and were collected within 10 days after symptom onset. Among negative HCWs, 6/190 (3.2%) seroconverted. Our data suggest that the determination of viral load can be used for appreciating the infectiousness of infected HCWs. These data could be helpful for facilitating their return to work.
