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Calheiros de Carvalho, Ana; De Camillis Rodrigues, Luiza; Ribeiro, Alany Ingrid; Fernandes da Silva, Maria Fátima das Graças; Soman de Medeiros, Lívia; Moura Veiga, Thiago André
Molecules (Basel, Switzerland), 12/2019, Volume: 25, Issue: 1Journal Article
In natural product studies, the purification of metabolites is an important challenge. To accelerate this step, alternatives such as integrated analytical tools should be employed. Based on this, the chemical study of (Rutaceae) was performed using two rapid dereplication strategies: (Bruker Daltonics , Bremen, Germany) MS data analysis combined with MS/MS data obtained from the GNPS platform. Through UHPLC-HRMS data, the first approach allowed, from crude fractions, a quick and visual identification of compounds already reported in the genus. Aside from this, by grouping compounds according to their fragmentation patterns, the second approach enabled the detection of eight molecular families, which presented matches for acridonic alkaloids, phenylacrylamides, and flavonoids. Unrelated compounds for have been isolated and characterized by NMR experiments, Lansamide I, Lansiumamide B, Lansiumamide C, and -(2-phenylethyl)cinnamamide.
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