Marine microalgae and cyanobacteria are sources of diverse bioactive compounds with potential biotechnological applications in food, feed, nutraceutical, pharmaceutical, cosmetic and biofuel industries. In this study, five microalgae, sp. S5, D1, sp. D3, sp. Z3 and sp. C6, and the cyanobacterium sp. C1 were isolated from the Adriatic Sea and characterized regarding their growth kinetics, biomass composition and specific products content (fatty acids, pigments, antioxidants, neutral and polar lipids). The strain sp. D3, showing the highest specific growth rate (0.009 h ), had biomass productivity of 33.98 ± 0.02 mg L day . Proteins were the most abundant macromolecule in the biomass (32.83-57.94%, g g ). D1 contained significant amounts of neutral lipids (68.36%), while the biomass of sp. D3, sp. Z3, sp. C6 and sp. C1 was rich in glycolipids and phospholipids (75%). The lipids of all studied microalgae predominantly contained unsaturated fatty acids. Carotenoids were the most abundant pigments with the highest content of lutein and neoxanthin in representatives of Chlorophyta and fucoxanthin in strains belonging to the Bacillariophyta. All microalgal extracts showed antioxidant activity and antimicrobial activity against Gram-negative and and Gram-positive .