The didactic knowledge of content (DKC) represents a theoretical category of the research of teaching that involves knowledge, allowing the teacher to make content teachable. The purpose of the present study aims to assess the behavior of DKC in the teaching of the "Scale-up of Chromatographic Columns" in the Unitary Operations in Bioprocess subject of the Chemical Engineering career, identifying difficulties in the comprehension of this subject by the students. The perceptive/intuitive, contextual, empirical and rational zones of the Model of Conceptual Profile were evaluated, resulting in an epistemological comparison among them, where two profiles were similar and two others were different, in correspondence with the different styles of teaching.