Initial geometric imperfections are unavoidable in steel members and frames due to erection and manufacturing tolerances. These include frame out-of-plumbness, member out-of-straightness and cross-sectional imperfections, and can have a significant influence on the response and resistance of steel structures. Thus, they need to be accounted for in the analysis and design of steel structures, especially when advanced design procedures are adopted. One of the easiest approaches to introduce geometric imperfections in structural finite element models is through the linear superposition of scaled eigenmodes, which are obtained from a priori elastic buckling analysis. Although the shape and magnitude of frame and member imperfections are specified in international standards, the rules for the combination of different types and directions of imperfections are unclear or impractical, and often require designers to consider many possible combinations to find the critical, or “worst case”, shape of the imperfection including the direction of each eigenmode. This paper investigates the influence of the direction of modes contributing to the imperfection on the ultimate load (i.e., resistance) of steel frames when using advanced analysis. Ultimate loads are estimated from advanced finite element simulations for 20 regular and irregular unbraced frames featuring steel and austenitic stainless steel compact sections, in which initial imperfections are modelled as linear superpositions of six scaled buckling modes considering all possible combinations of direction. The results show that the influence of the imperfection direction on the ultimate frame load is small, and that assuming a combination of all buckling modes with positive amplitudes provides a simple and accurate estimation of the critical imperfection combination. Display omitted •Influence of the imperfection direction on the strength of steel frames is investigated.•Imperfections are modelled as a linear superposition of six scaled buckling modes.•Different regular and irregular steel and stainless steel frames with compact sections are examined.•Ultimate load factors for all possible direction combinations are obtained and compared.•The notion of worst case imperfection is challenged.