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Li, Juan; Chen, Like; Liang, Jing; Xu, Rongfang; Jiang, Yingli; Li, Yizhen; Ding, Jian; Li, Min; Qin, Ruiying; Wei, Pengcheng
Genome Biology, 07/2022, Volume: 23, Issue: 1Journal Article
Abstract Low efficiency has seriously restricted the application of prime editing (PE) systems in plants. In this study, we develop an enhanced plant prime editor 2 system, enpPE2, by stacking various optimization strategies, including updating the PE architecture to PEmax and expressing engineered pegRNA with a structured motif under the control of a composite promoter. In T 0 rice plants, enpPE2 exhibits editing frequencies of 64.58% to 77.08%, which are much higher than the frequencies with unmodified pPE2. Our results indicate that the enpPE2 system provides a robust and powerful tool for the precise modification of plant genomes.
