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Tóth, Gábor; Santa-Maria, Ana R.; Herke, Ibolya; Gáti, Tamás; Galvis-Montes, Daniel; Walter, Fruzsina R.; Deli, Mária A.; Hunyadi, Attila
Journal of natural products, 04/2023, Volume: 86, Issue: 4Journal Article
Ecdysteroid-containing herbal extracts, commonly prepared from the roots of Cyanotis arachnoidea, are marketed worldwide as a “green” anabolic food supplement. Herein are reported the isolation and complete 1H and 13C NMR signal assignments of three new minor ecdysteroids (compounds 2–4) from this extract. Compound 4 was identified as a possible artifact that gradually forms through the autoxidation of calonysterone. The compounds tested demonstrated a significant protective effect on the blood–brain barrier endothelial cells against oxidative stress or inflammation at a concentration of 1 μM. Based on these results, minor ecdysteroids present in food supplements may offer health benefits in various neurodegenerative disease states.
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