Abstract Background Although laparoscopic pancreatic resection (LPR) has become a routine, large single center series are still lacking. Our aim was to analyze the results of a large European single center series of LPR. Study Design Between January 2008 and September 2015, 300 LPR were performed and studied prospectively including 165 (55%) distal pancreatectomies, 68 (23%) pancreatoduodenectomies (PD), 30 (10%) enucleations, 35 (11%) central pancreatectomies and 2 (1%) total pancreatectomies. Results Mean age was 54 ± 15.4 years old (17-87) and most patients were women (58%). LPR was performed for malignancy (46%), low potential malignant (44%) or benign (10%) diseases. The mean operative duration was 211 ± 102 min (30-540) and 351± 59 (240-540) min for PD, and decreased with the learning curve. Mean blood loss was 229 ±269 ml (0-1500) and 13 patients (4%) were transfused. Conversion was required in 12 patients (4%), and only 5 in the last 250 patients (2% vs 14%, P<0.001). Mortality occurred in 4 (1.3%) patients and only after PD (5.8%). Common complications were pancreatic fistula (n=124, 41%), bleeding (n=35, 12%) and reoperation (n=28, 9%). The postoperative outcome was less favorable in procedures with a reconstruction phase (n=105) than in those without (n=195) with increased mortality (3.8% vs 0%, p=0.04), overall morbidity (76% vs % 52%, p<0.001) and mean hospital stay (26 ±15vs 16±10 days, p<0.001). Conclusions LPR without a reconstruction phase has an excellent outcome. LPR with a reconstruction phase, especially PD, has a less favorable outcome and further randomized studies are required to conclude on the safety and benefits of this approach.