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Utilization of interferometric light microscopy for the rapid analysis of virus abundance in a riverRoose-Amsaleg, Céline; Fedala, Yasmina; Vénien-Bryan, Catherine; Garnier, Josette; Boccara, Albert-Claude; Boccara, Martine
Research in microbiology, 06/2017, Volume: 168, Issue: 5Journal Article
There is a constant need for direct counting of biotic nanoparticles such as viruses to unravel river functioning. We used, for the first time in freshwater, a new method based on interferometry differentiating viruses from other particles such as membrane vesicles. In the French Marne River, viruses represented between 42 and 72% of the particles. A spring monitoring in 2014 revealed their increase (2.1 × 107 to 2.1 × 108 mL−1) linked to an increase in algal biomass and diversity of bacterial plankton. Predicted virus size distributions were in agreement with transmission electron microscopy analysis suggesting a dominance of large viruses (≥60 nm).
