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Talay, Mustafa; Richman, Ethan B.; Snell, Nathaniel J.; Hartmann, Griffin G.; Fisher, John D.; Sorkaç, Altar; Santoyo, Juan F.; Chou-Freed, Cambria; Nair, Nived; Johnson, Mark; Szymanski, John R.; Barnea, Gilad
Neuron (Cambridge, Mass.), 11/2017, Volume: 96, Issue: 4Journal Article
Mapping neural circuits across defined synapses is essential for understanding brain function. Here we describe trans-Tango, a technique for anterograde transsynaptic circuit tracing and manipulation. At the core of trans-Tango is a synthetic signaling pathway that is introduced into all neurons in the animal. This pathway converts receptor activation at the cell surface into reporter expression through site-specific proteolysis. Specific labeling is achieved by presenting a tethered ligand at the synapses of genetically defined neurons, thereby activating the pathway in their postsynaptic partners and providing genetic access to these neurons. We first validated trans-Tango in the Drosophila olfactory system and then implemented it in the gustatory system, where projections beyond the first-order receptor neurons are not fully characterized. We identified putative second-order neurons within the sweet circuit that include projection neurons targeting known neuromodulation centers in the brain. These experiments establish trans-Tango as a flexible platform for transsynaptic circuit analysis. •A genetic approach for transsynaptic tracing and manipulation of neural circuits•Genetic access to neurons based on their connectivity•Glomerulus-specific patterns of second-order neurons in the fly olfactory system•Identifying second-order gustatory neurons and their target areas in the fly brain Talay and Richman et al. develop a genetic method for transsynaptic labeling of neural circuits in Drosophila. They validate it in the olfactory system and implement it in the gustatory system to reveal second-order projections of sweet tastant-responsive neurons.
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