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Xu, C Shan; Pang, Song; Shtengel, Gleb; Müller, Andreas; Ritter, Alex T; Hoffman, Huxley K; Takemura, Shin-Ya; Lu, Zhiyuan; Pasolli, H Amalia; Iyer, Nirmala; Chung, Jeeyun; Bennett, Davis; Weigel, Aubrey V; Freeman, Melanie; van Engelenburg, Schuyler B; Walther, Tobias C; Farese, Jr, Robert V; Lippincott-Schwartz, Jennifer; Mellman, Ira; Solimena, Michele; Hess, Harald F
Nature, 11/2021, Volume: 599, Issue: 7883Journal Article
Understanding cellular architecture is essential for understanding biology. Electron microscopy (EM) uniquely visualizes cellular structures with nanometre resolution. However, traditional methods, such as thin-section EM or EM tomography, have limitations in that they visualize only a single slice or a relatively small volume of the cell, respectively. Focused ion beam-scanning electron microscopy (FIB-SEM) has demonstrated the ability to image small volumes of cellular samples with 4-nm isotropic voxels . Owing to advances in the precision and stability of FIB milling, together with enhanced signal detection and faster SEM scanning, we have increased the volume that can be imaged with 4-nm voxels by two orders of magnitude. Here we present a volume EM atlas at such resolution comprising ten three-dimensional datasets for whole cells and tissues, including cancer cells, immune cells, mouse pancreatic islets and Drosophila neural tissues. These open access data (via OpenOrganelle ) represent the foundation of a field of high-resolution whole-cell volume EM and subsequent analyses, and we invite researchers to explore this atlas and pose questions.
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