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Yao, Wantian; Kong, Lingling; Lei, Diya; Zhao, Bing; Tang, Honglan; Zhou, Xuan; Lin, Yuanxiu; Zhang, Yunting; Wang, Yan; He, Wen; Li, Mengyao; Chen, Qing; Luo, Ya; Wang, Xiaorong; Tang, Haoru; Zhang, Yong
Frontiers in plant science, 07/2023, Volume: 14Journal Article
The all-red A. arguta ( Actinidia arguta ) is an anthocyanin-rich and excellent hardy fruit. Many studies have focused on the green-fleshed A. arguta , and fewer studies have been conducted on the all-red A. arguta . Here we reported a regeneration and Agrobacterium-mediated transformation protocol by using leaves of all-red A. arguta as explants. Aseptic seedling leaves of A. arguta were used as callus-inducing materials. MS medium supplemented with 0.3 mg·L -1 2,4-D and 1.0 mg·L -1 BA was the optimal medium for callus induction of leaves, and medium supplemented with 3 mg·L -1 tZ and 0.5 mg·L -1 IAA was optimal for adventitious shoot regeneration. The best proliferation medium for adventitious buds was MS + 1.0 mg·L -1 BA + 0.3 mg·L -1 NAA. The best rooting medium was 1/2MS + 0.7 mg·L -1 IBA with a 100% rooting rate. For the red flesh hardy kiwi variety ‘Purpurna Saduwa’ ( A. arguta var. purpurea ), leaves are receptors for Agrobacterium (EHA105)-mediated transformation. The orthogonal experiment was used for the optimization of each genetic transformation parameter and the genetic transformation of the leaves was 21% under optimal conditions. Our study provides technical parameters for applying genetic resources and molecular breeding of kiwifruit with red flesh.
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