To investigate the resistance to bacterial adhesion of materials used in oculoplastic surgery, particularly materials used in the manufacture of orbital implants. Seven organisms of conjunctival flora (two strains of and one strain each of , , , , and ) were selected. A lactic acid bacterium ( ) was also included as positive control because of its well-known adhesion ability. Eight materials used to make oculoplastic prostheses were selected (glass, steel, polytetrafluoroethylene, polymethylmethacrylate, silicone from orbital implants, commercial silicone, porous polyethylene, and semi-smooth polyethylene). Materials surfaces and biofilms developed by strains were observed by scanning electron microscopy. Kinetics of growth and adhesion of bacterial strains were determined by spectrophotometry. Each strain was incubated in contact with plates of the different materials. After growth, attached bacteria were re-suspended and colony-forming units (CFUs) were counted. The number of CFUs per square millimetre of material was statistically analyzed. A mature biofilm was observed in studied strains except , which simply produced a microcolony. Materials showed a smooth surface on the microbial scale, although steel exhibited 1.0-µm-diameter grooves. Most organisms showed significant differences in adhesion according to the material. There were also significant differences in the total number of CFUs per square millimetre from each material ( =0.044). CFU counts were significantly higher in porous polyethylene than in silicone from orbital implants ( =0.038). Silicone orbital implants can resist microbial colonization better than porous polyethylene implants.