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Hans, Lucia; Steegen, Kim; Ketseoglou, Irene; Mahlumba, Zukiswa; Cassim, Naseem; Wiggill, Tracey; Venter, W.D. Francois; Stevens, Wendy
International journal of infectious diseases, 09/2021, Volume: 110Journal Article
•Rapid transition to automated SARS-CoV-2 molecular tests was challenging.•Adapted protocols for verification of assays used.•Continuous management of human resources required.•Improved workflow reduced time to obtaining a result.•Strategies executed increased percentage of samples reported within turnaround-time. Africa’s readiness to respond to the SARS-COV-2 pandemic was tested due to reliance on rapid turn-around-time of polymerase chain reaction results for clinical management, isolation and quarantine decisions. The NHLS HIV Molecular Laboratory in Johannesburg, South Africa, is one of the largest automated HIV molecular laboratories worldwide. Despite its extensive molecular capacity and experience in managing high volumes acquired from a large HIV program, significant challenges were encountered during its rapid transition to large scale SARS-CoV-2 testing. We describe the strategies employed to manage these challenges that resulted in a 30% improvement in SARS-CoV-2 test turn-around-time during the first wave peak during which approximately 25000 samples were tested per month, and further improvement during the second wave peak, with 81% within targeted turn-around-time.
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