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Li, Zhong; Zhang, Lilan; Xu, Kangwei; Jiang, Yuanyuan; Du, Jieke; Zhang, Xingwang; Meng, Ling-Hong; Wu, Qile; Du, Lei; Li, Xiaoju; Hu, Yuechan; Xie, Zhenzhen; Jiang, Xukai; Tang, Ya-Jie; Wu, Ruibo; Guo, Rey-Ting; Li, Shengying
Nature communications, 07/2023, Volume: 14, Issue: 1Journal Article
Diterpene synthase VenA is responsible for assembling venezuelaene A with a unique 5-5-6-7 tetracyclic skeleton from geranylgeranyl pyrophosphate. VenA also demonstrates substrate promiscuity by accepting geranyl pyrophosphate and farnesyl pyrophosphate as alternative substrates. Herein, we report the crystal structures of VenA in both apo form and holo form in complex with a trinuclear magnesium cluster and pyrophosphate group. Functional and structural investigations on the atypical DSFVSD motif of VenA, versus the canonical Asp-rich motif of DDXX(X)D/E, reveal that the absent second Asp of canonical motif is functionally replaced by Ser116 and Gln83, together with bioinformatics analysis identifying a hidden subclass of type I microbial terpene synthases. Further structural analysis, multiscale computational simulations, and structure-directed mutagenesis provide significant mechanistic insights into the substrate selectivity and catalytic promiscuity of VenA. Finally, VenA is semi-rationally engineered into a sesterterpene synthase to recognize the larger substrate geranylfarnesyl pyrophosphate.
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