This study aims to identify suitable lactobacilli that have anti-carbapenem-resistant (CRE) activity with tolerance to pepsin and bile salts. Fifty-seven spp. strains encompassing nine species were collected for investigation. Their viabilities in the presence of pepsin and bile salts were tested using tolerance tests. Their anti-CRE effects were assessed by agar well diffusion and broth microdilution assay, as well as time-kill test. Of the 57 isolates collected, 31 had a less than 2-log reduction in their viability in both pepsin and bile salt tolerance tests. Of these 31 isolates, 5 (LUC0180, LUC0219, LYC0289, LYC0413, and LYC1031) displayed the greatest anti-CRE activity with a CRE zone of inhibition greater than 15 mm in agar well diffusion assays. The minimal inhibitory percentages of supernatants from these five strains against CREs ranged from 10 to 30%. With the exception of LUC0180, which had a minimal bactericidal percentage ≥ 40%, the bactericidal percentage of all the strains ranged from 20 to 40%. The inhibitory effect of the cell-free culture supernatants from these strains did not change after heating but was abolished as the pH changed to 7.0. After a 24-h incubation, five of the strains at a concentration of 10 CFU/ml totally inhibited the growth of carbapenem-resistant (CRE316) and (CRE632). After a 48-h incubation, the growth of CRE316 was completely inhibited under each concentration of lactobacilli based on time-kill test. Furthermore, when the concentration of lactobacilli was at 10 CFU/ml, the decline in pH was faster than at other concentrations. Some strains exhibit anti-CRE activity, which suggests potential applications for controlling or preventing CRE colonization or infection.