Land surface temperature (LST) predictors, such as impervious and vegetated surfaces, strongly influence the urban landscape mosaic, also changing microclimate conditions and exacerbating the surface urban heat island (SUHI) phenomenon. The aim of this study was to investigate the summer daytime SUHI phenomenon and the role played by impervious and tree cover surfaces in the 10 Italian peninsular metropolitan cities. Summer daytime LST values were assessed by using MODIS data referred to the months of June, July and August from 2016 to 2018. High spatial resolution (10 m) of impervious surface and tree cover layers was calculated based on open-data developed by the Italian National Institute for Environmental Protection and Research. A novel informative urban surface landscape layer was developed combining impervious surfaces and tree cover densities and its mapping for metropolitan cities was performed. Summer daytime SUHI rose significantly, increased especially in inland cities, by increasing the size of areas with low tree cover densities in the metropolitan core (or decreasing areas with low tree cover densities outside the metropolitan core), further increasing its intensity when the impervious density grew. A mitigating effect of the sea on daytime LST and SUHI was observed on coastal cities. The most intense SUHI phenomenon was observed in Turin (the largest Italian metropolitan city): for every 10% increase in areas with highly impervious surfaces and low tree cover densities in the metropolitan core, the SUHI significantly (p < 0.001) increased by 4.0 °C. Increased impervious surfaces combined with low tree cover densities represented the main driving process to increase the summer daytime SUHI intensity in most studied cities. These findings are useful to identify summer daytime LST critical areas and to implement the most efficient urban-heat-island mitigation strategies in order to safeguard the vulnerable urban environment and enhance quality of life for the population. Display omitted •The study of processes that regulate the urban microclimate is a priority.•The different combinations of impervious and vegetated surfaces need to be studied.•A new urban surface landscape layer based on open-source data was developed.•SUHI was higher in inland and the largest metropolitan cities than in coastal ones.•Mitigation actions must be well coordinated and based on metropolitan-city features.