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  • Subpopulations of human granulosa-luteal cells in natural and stimulated in vitro fertilization-embryo transfer cycles
    Geršak, Ksenija ; Tomaževič, Tomaž
    OBJECTIVE: To find out the differences between human granulosa-luteal cells derived from natural and stimulated IVF-ET cycles. DESIGN: Cells were obtainedfrom dominant follicles of 52 women with ... natural cycles in whom preovulatory hCG was given when the follicle was mature and from 50 dominant follicles of women undergoing IVF-ET with hMG and hCG. SETTING: In Vitro Fertilization Unit, University Department of Obstetrics and Gynecology Ljubljana, Slovenia. MAIN OUTCOME MEASURE: Four subpopulations of cells were observed by computerized image analysis in which hCG was localized using immunoperoxidase staining. RESULTS: The nonluteinized granulosa cells from natural cycles were larger than those from the stimulated ones. In luteinized cell types, there was no difference in cell area between natural and stimulated cycles. Three staining types of hCG localization were found: on thesurface membrane, on the surface membrane and within the cytoplasm, and within the cytoplasm alone. The hCG stained cells from natural cycles were larger than the ones from stimulated cycles. The natural developing follicles contained more hCG stained cells than the stimulated ones. The follicles with fertilizable oocytes had more cells with cytoplasmic hCG localization. Only innatural cycles was there was a correlation between follicular fluid hCG levels and the percentage of the hCG stained cells. CONCLUSION: We found differences in morphometric characteristics and hCG localization between humangranulosa and granulosa-luteal cells obtained from natural and stimulatedIVF-ET cycles.
    Vir: Fertility and sterility. - ISSN 0015-0282 (Letn. 65, št. 3, 1996, str. 608-613)
    Vrsta gradiva - članek, sestavni del
    Leto - 1996
    Jezik - angleški
    COBISS.SI-ID - 11516889

vir: Fertility and sterility. - ISSN 0015-0282 (Letn. 65, št. 3, 1996, str. 608-613)
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