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  • Brefeldin A and a synthetic peptide to ADP-ribosylation factor (ARF) inhibit regulated exocytosis in melanotrophs
    Rupnik, Marjan ...
    We investigated the role of ADP-ribosylation factor (ARF) in regulated exocytosis in patch-clamped rat melanotrophs. Addition of brefeldin A (BFA) toinhibit activation of endogenous ARF protein was ... found to attenuate regulated secretory activity monitored as changes in membrane capacitance (Cm). A synthetic peptide to amino acids 46-61 of ARF (P-14) was also found toinhibit Ca(2+)-induced secretory activity in these cells. This inhibition was not apparent with a scrambled amino acid sequence of ARF-P14 peptide. Thispaper provides the first patch-clamp study to suggest that the small GTP-binding protein ARF is required to trigger release of secretory granules from rat pituitary melanotrophs.
    Vir: Neuroreport. - ISSN 0959-4965 (Let. 6, št. 6, 1995, str. 853-856)
    Vrsta gradiva - članek, sestavni del
    Leto - 1995
    Jezik - angleški
    COBISS.SI-ID - 13273

vir: Neuroreport. - ISSN 0959-4965 (Let. 6, št. 6, 1995, str. 853-856)
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