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  • Fluorescence measurements of cytosolic calcium: combined photometry with electrophysiology
    Mason, W... T. ...
    The development of optical probes for biological activity combined with powerful photon aquisition and analysis technology is having a major effect onthe study of electrophysiology parameters of ... single living cells. The best of these probes appear not to interfere with normal cellular processes, although they do rquire caution in interpretation of data, because the probes may localize within cells, rendering them insensitive or modifying their properties.In thes case of ions, photometric technology allows the applicationof these probes to yield data that will shed light on the relationship between ion entry and ionic concentration inside the cell on veryfast time scales. New real-time imaging probes for ions, cyclic mucleotide, cellular enzymes and genetic material are under development and appear to be suitable for work on living cells, all of which will further extend our capability to study cellular homeostasis and its perturbation in health and disease states. A variety of commercial systems are available, making entry into this area of research both feasible and cost-effective.
    Vir: Methods in neurosciences. - ISSN 1043-9471 (Let. 27, 1995, str. 81-122)
    Vrsta gradiva - članek, sestavni del
    Leto - 1995
    Jezik - angleški
    COBISS.SI-ID - 13529

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