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  • Nicardipine enantiomers inhibit calcium and outward currents in rat pars intermedia cells
    Kocmur, Laura ; Zorec, Robert, 1958-
    The whole-cell patch clamp technique was used to investigate the effect of thedihydropyridine antagonist nicardipine and its two enantiomers on calcium and outward currents in rat pars intermedia ... cells. Inward voltage-activated calcium currents as well as outward currents were blocked by nicardipines (enantiomers and racemic substance). We found a highly significant correlationbetween the blockage of voltage-activated currents and outward currents, indicating an association between recorded currents. Therefore, the inhibitory effect of nicardipines was measured as outward current inhibition. The effects of (-) and (+) enantiomers as well as the racemic nicardipine wereconcentration-dependent. The (+)-enantiomer was the least potent with an ED50 of 3.8 +- 1.2 micro M, white the (-)-enantiomer inhibited currents with an ed50 of 0.7 micro M. Racemic nicardipine blocked currents with an ed50 of 2.2 +- 0.6 micro M.
    Vir: Neuroscience letters. - ISSN 0304-3940 (Letn. 207, št. 2, 1996, str. 121-124)
    Vrsta gradiva - članek, sestavni del
    Leto - 1996
    Jezik - angleški
    COBISS.SI-ID - 14041

vir: Neuroscience letters. - ISSN 0304-3940 (Letn. 207, št. 2, 1996, str. 121-124)
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