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Cyclooxygenase-2 expression in thyroid nodulesSpecht, Michelle C. ...Factors contributing to the development of thyroid neoplasia remain poorly understood. Recent evidence indicates that overexpression of the inducible cyclooxygenase, COX-2, is important in the ... pathogenesis of epithelial carcinomas. These studies were undertaken to evaluate whether COX-2 is upregulated in human thyroid neoplasia. Benign (n=14), and malignant (n=14) thyroid nodules were analyzed for expression of COX-2 mRNA by quantitative RT-PCR. Immunoblotting and immunohistochemistry were performed on representative samples. Three human thyroid cancer cell lines were similarly analyzed for COX-2 expression. Levels of COX-2 mRNA were significantly increased in thyroid nodule samples compared with adjacent tissue in the malignant specimens but not in the benign specimens. Additionally, COX-2 mRNA levels were significantly increased in malignant nodule samples compared with benign nodule samples. COX-2protein expression was higher in 8 of 10 thyroid nodules compared with the adjacent tissue. Immunohistochemical analysis localized expression of COX-2 to the malignant epithelialcells. Immunoflurescence demonstrated COX-2 protein expression in all three thyroid cell lines. Finally, COX-2 expression could be detected by RT-PCR in fine needle aspiration specimens of thyroid nodules. These data indicate that COX-2is up-regulated in human thyroid cancer, but not in benign thyroid nodules, and suggest that COX-2 expression may serve as a marker of malignancyin thyroid nodules.Vir: The Journal of clinical endocrinology & metabolism. - ISSN 0021-972X (Letn. 87, št. 1, 2002, str. 358-363)Vrsta gradiva - članek, sestavni delLeto - 2002Jezik - angleškiCOBISS.SI-ID - 15058905
Avtor
Specht, Michelle C. |
Tucker, Olga N. |
Hočevar, Marko, 1964- |
Gonzalez, Donald |
Teng, Lisong |
Fahey, Thomas J., 3rd
Teme
Thyroid nodule |
Enzymology |
Etiology |
Pathology |
Prostaglandin-endoperoxide synthase |
Biosynthesis |
Metabolism |
Isoenzymes |
Genetics |
Metabolism |
Gene expression regulation, enzymologic |
Tumor markers, biological |
Analysis |
Tumor cells, cultured |
Polymerase chain reaction |
RNA, messenger |
Analysis |
Phorbol esters |
Immunohistochemistry |
Biopsy, needle |
Epithelium |
Enzymology |
Gensko izražanje, regulacija encimska |
Izoencimi |
Prostaglandin-endoperoksidna sintaza |
Ščitnični vozel |
Biopsija igelna |
Epitelij |
Forbol estri |
Imunohistokemija |
Polimeraza, verižna reakcija |
RNA, prenašalna |
Tumorske celične kulture |
Tumorski markerji, biološki
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Hočevar, Marko, 1964- | 12023 |
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