Akademska digitalna zbirka SLovenije - logo
VSE knjižnice (vzajemna bibliografsko-kataložna baza podatkov COBIB.SI)
  • Cyclooxygenase-2 expression in thyroid nodules
    Specht, Michelle C. ...
    Factors contributing to the development of thyroid neoplasia remain poorly understood. Recent evidence indicates that overexpression of the inducible cyclooxygenase, COX-2, is important in the ... pathogenesis of epithelial carcinomas. These studies were undertaken to evaluate whether COX-2 is upregulated in human thyroid neoplasia. Benign (n=14), and malignant (n=14) thyroid nodules were analyzed for expression of COX-2 mRNA by quantitative RT-PCR. Immunoblotting and immunohistochemistry were performed on representative samples. Three human thyroid cancer cell lines were similarly analyzed for COX-2 expression. Levels of COX-2 mRNA were significantly increased in thyroid nodule samples compared with adjacent tissue in the malignant specimens but not in the benign specimens. Additionally, COX-2 mRNA levels were significantly increased in malignant nodule samples compared with benign nodule samples. COX-2protein expression was higher in 8 of 10 thyroid nodules compared with the adjacent tissue. Immunohistochemical analysis localized expression of COX-2 to the malignant epithelialcells. Immunoflurescence demonstrated COX-2 protein expression in all three thyroid cell lines. Finally, COX-2 expression could be detected by RT-PCR in fine needle aspiration specimens of thyroid nodules. These data indicate that COX-2is up-regulated in human thyroid cancer, but not in benign thyroid nodules, and suggest that COX-2 expression may serve as a marker of malignancyin thyroid nodules.
    Vir: The Journal of clinical endocrinology & metabolism. - ISSN 0021-972X (Letn. 87, št. 1, 2002, str. 358-363)
    Vrsta gradiva - članek, sestavni del
    Leto - 2002
    Jezik - angleški
    COBISS.SI-ID - 15058905

Vnos na polico