Akademska digitalna zbirka SLovenije - logo
VSE knjižnice (vzajemna bibliografsko-kataložna baza podatkov COBIB.SI)
  • Naravni plazmid pRK100 bakterije Escherichia coli je sestavljen kot mozaik
    Starčič Erjavec, Marjanca ...
    Mosaicism of the Large Natural Escherichia coli Plasmid pRK100 - In search forthe evolutionary origin of the conjugative plasmid pRK100, we determined the plasmid's functional replication regions and ... performed targeted genetic analysis of the plasmid. Construction of minireplicons via ligation of Tn1725 with plasmid fragments and targeted cloning of putative replication regions, followed by sequence analysis indicated two functional replication regions, a plasmid F-like RepFIB and a plasmid R1-like RepFIIA replication region. Partial nucleotide sequencing of regions of the plasmid revealed genes that encode a putative enterochelin iron uptake system previously associated with an Escherichia coli pathogenicity island, PAI III536, and the pColV-like aerobactin genes. In addition, a homologue of the plasmid R100-like rmoA gene was found. PCR and hybridisation experiments further demonstrated that pRK100 harbours IS2 and IS3 insertion sequences that may have facilitated in the acquisition of elements from other DNA molecules. These data together with theprevious identification of a F-like tra region and a pColIa-like colicin Ia, indicate that pRK100 has a highly mosaic structure with elements derived from many different known large natural plasmids, and also from chromosome.
    Vir: Raziskovalno delo podiplomskih študentov v Sloveniji - ena znanost [Elektronski vir] : e-zbornik (Str. 395-404)
    Vrsta gradiva - članek, sestavni del
    Leto - 2003
    Jezik - slovenski
    COBISS.SI-ID - 16484313

Vnos na polico