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  • Uporaba molekularnih metod za določanje bakterije povzročiteljice obročkaste gnilobe krompirja = The use of molecular biology methods for detection of potato ring rot disease
    Demšar, Tina, 1973- ...
    Clavibacter michiganensis (Smith) Davis et aL ssp. sepedonicus (Spieckermamm et Kotthoff) Davis et aL is the causal agent of bacterial ring rot disease of potato. Bacteria is listed on LA.II. List of ... harmful organisms in Slovenia. Diagnostic of bacteria in our laboratory is conducted as survey in the frame of official plant protection service. It was not yet found in Slovenia, but wehad identified bacterium in imported ware potato for the first time in year 2001. A testing procedure according to EU directive (93/85/ECC) is based on screening serological immunofluoresce or ELISA test. If serological test on potato extract is positive, inoculation of test plants Solanum melongena cv. Black Beauty is pertormed. From inoculated test plants bacteria is then isolated on medium and confirmed with different biochemical tests and molecular methods. In our laboratory different molecular methods for detectionof ring rot disease in potato extracts and confirmation of bacterial isolates are used: PCR, Real Time PCR and FISH (fluorescent in situ hybridization).
    Vir: Zbornik predavanj in referatov 6. slovenskega posvetovanja o varstvu rastlin, Zreče, 4.-6. marec 2003 = Lectures and papers presented at the 6th Slovenian Conference on Plant Protection, Zreče, 4.-6. March 2003 (Str. 118-122)
    Vrsta gradiva - prispevek na konferenci
    Leto - 2003
    Jezik - slovenski
    COBISS.SI-ID - 16485849

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