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  • Simple and cost-effective isolation of monocytes from buffy coats
    Repnik, Urška ; Knežević, Miomir ; Jeras, Matjaž
    We have optimized the procedure of monocyte isolation on a Percoll density gradient. The new procedure consists of three steps: (1) the isolation of MNC on a Ficoll density gradient; (2) the ... separation of monocytes from lymphocyteson a high-density hyper-osmotic Percoll density gradient; and (3) the separation of monocytes from platelets and dead cells on a low-density iso-osmotic Percoll density gradient. The procedure is simple and cost-effective. Monocyte purity and recovery are both about 75% and platelet contamination is low. The isolated monocytes retain their capacity to differentiate into dendritic cells in vitro.
    Vir: Journal of Immunological Methods. - ISSN 0022-1759 (Vol. 278, no. 1/2, 2003, str. 283-292)
    Vrsta gradiva - članek, sestavni del
    Leto - 2003
    Jezik - angleški
    COBISS.SI-ID - 16748505

vir: Journal of Immunological Methods. - ISSN 0022-1759 (Vol. 278, no. 1/2, 2003, str. 283-292)
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