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  • Prepoznavanje značilnih kromosomskih prireditev z reagenčnim kompletom mDx HemaVision pri bolnikih z levkemijo = Detection of specific chromosomal rearrangement in leukemia patients by mDx HemaVision kit
    Pajič, Tadej ; Ljutić, Elvira ; Černelč, Peter
    Background. The mDx HemaVision kit is a qualitative multiplex and nested Reverse Transcription. Polymerase Chain Reaction (RTPCR) test designed to detect 28 different tra`aslocations or chromosomal ... rearrangement, found to be specific for particular subtypes of leukemia. The presen.ce or absence of the specific mRNA transcripts or cDNA segments after the reverse transcription of the fusion or abnormal genes, appeared after chromosomal rearrangements, couldbe determined by the kit. Patients and methods. The usefulness of the kitwas tested on the 26 RNA sarnples of patients with acute leukemia and sevenpatients with chronic mieloproliferative diseases and by comparison of the results between mDx HemaVision kit and standardized RT-PCR protocol for the specific mRNA transcripts of the t(9;22)(q34;q11), t(8;21)(q22;q22), t(15;17)(q22;q22) and t(4;11)(q21;q23). The RNA samples were isolated from mononuclear cells of the bone marrow after Ficoll-Paque density gradient centrifugation and with a High Pure RNA isolation kit. The cDNA synthesis and Polymerase Chain Reaction were performed as described in mDx HemaVisoin's or standardized RT-PCR's protocols. The PCR products were analyzed by agarose gelelectrophoresis, by staining with etidium bromide and by visualization under UV light. Results. We obtained 100% concordance of the results by both methods. Specific BCR-ABL mRNA transcripts were found in four chronic myeloid leukemia patients, one in B acute lymphoblastic leukemia (B-ALL) and one with bifenotypic leukemia (BAL) patient. AML1-ETO mRNA transcript of the t(8;21)(q22;q22) was identified in two patients with acute myeloid leukemia (AML). The CBFbeta/MYH11 mRNA transcript specific for invl6(p13;q22) was obtained in AML patient with abnormal eozinophiles in bone marrow. MLL/AF4 mRNA transcript of the t(4;11)(q21;q23) was found in the girl with BALL and inpatient with B-ALL after treatment. (Abstract truncated at 2000 characters).
    Vir: Številka ob 2. kongresu hematologov in transfuziologov Slovenije z mednarodno udeležbo (str. I-9-I-12)
    Vrsta gradiva - prispevek na konferenci ; neleposlovje za odrasle
    Leto - 2004
    Jezik - slovenski
    COBISS.SI-ID - 17634265

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