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Molecular characterization of genes encoding cytosolic Hsp70s in the zygomycete fungus Rhizopus nigricansČernila, Boštjan ; Črešnar, Bronislava, 1950-2024 ; Breskvar, KatjaPrevious studies have shown that some stressors, including steroid hormones 21-OH progesterone and testosterone, stimulate the accumulation of heat shock protein 70 (hsp70) messenger ribonucleic acid ... (mRNA) population in the zygomycete filamentous fungus Rhizopus nigricans. In thls study we report the cloning of 3 R nigricans hsp70 genes (Rnhsp70-1, Rnhsp70-2, and Rnhsp70-3) encoding cytosolic Hsp70s. With a Southern blot experiment under high stringency conditions we did not detect any additional highly homologous copies of the cytosolic hsp70 genes in the R nigricans genome. Sequence analyses showed that all 3 genes contain introns within the open reading frame. The dynamics of the R nigricans molecular response to progesterone, 21-OH progesterone, and testosterone, as well as to heat shock, copper ions, hydrogen peroxide, and ethanol was studied by temporal analysls of Rnhsp70-i and Rnhsp70-2 mRNA accumulation. Northern blot experiments revealed that the Rnhsp70-2 transcript level Is not affected by testosterone, whereas mRNA levels of both genes are rapidly increased with all the other stressors studied. Moreover, the decrease of transcript levels is notably delayed in ethanol stress, and a difference is observed between the profiles of Rnhsp70-iand Rnhsp70-2 transcripts during heat stress.Vir: Cell stress & chaperones. - ISSN 1355-8145 (Letn. 8, št. 4, 2003, str. 317-328)Vrsta gradiva - članek, sestavni delLeto - 2003Jezik - angleškiCOBISS.SI-ID - 17664473
Avtor
Černila, Boštjan |
Črešnar, Bronislava, 1950-2024 |
Breskvar, Katja
Teme
Rhizopus |
Genetics |
Heat-Shock Proteins 70 |
Genome, Fungal |
Genomic Library |
Cloning, Molecular |
Dna, Complementary |
Testosterone |
Desoxycorticosterone |
Progesterone |
Cytosol |
Introns |
Rna, Messenger |
Blotting, Northern |
Blotting, Southern |
Genom gliv |
Genomska knjižnica |
Kloniranje molekulsko |
Rhizopus |
Vročinski šok, beljakovine 70 |
Blot, Northern |
Blot, Southern |
Citosol |
DNA komplementarna |
Deoksikortikosteron |
Introni |
Progesteron |
RNA, prenašalna |
Testosteron
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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Černila, Boštjan | 19879 |
Črešnar, Bronislava, 1950-2024 | 08777 |
Breskvar, Katja | 03079 |
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