VSE knjižnice (vzajemna bibliografsko-kataložna baza podatkov COBIB.SI)
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Studying cell death initiation using a digital microscope [Elektronski vir]Arnšek, Tina ...Hypersensitive response (HR)-conferred resistance is an effective defense response that can be determined by the N resistance genes. HR is manifested as the formation of cell death zones on ... inoculated leaves. Here, a protocol for studying the rate of cell death initiation by imaging inoculated leaves in the time between the cell death initiation and the cell death appearance using a digital microscope is presented. The digital microscope enables a continuous imaging process in desired intervals, which allows an accurate determination of cell death initiation rate up to minutes exactly, as opposed to hours in traditional methods. Imaging with the digital microscope is also independent of light and can therefore be used during day and night without disturbing the circadian rhythm of the plant. Different pathosystems resulting in programmed cell death development could be studied using this protocol with minor modifications. Overall, the protocol thus allows simple, accurate, and inexpensive identification of cell death initiation rate.Vir: Journal of visualized experiments [Elektronski vir]. - ISSN 1940-087X (Vol. 201, [article no.] 3027, 2023, str. 1-9)Vrsta gradiva - e-članek ; neleposlovje za odrasleLeto - 2023Jezik - angleškiCOBISS.SI-ID - 177346819
Digitalne vsebine: dCOBISS
| Avtor | Arnšek, Tina ... |
| Naslov | Studying cell death initiation using a digital microscope [Elektronski vir] |
| Datum objave | 2023-11-10 |
| COBISS.SI-ID | 177346819 |
| Verzija objave v repozitoriju | Založnikova različica |
| Licenca objave v repozitoriju | Creative Commons Priznanje avtorstva-Nekomercialno-Brez predelav 3.0 Nedoločena |
| Embargo | Takojšnja javna objava |
Projekti, iz katerih je bila financirana objava
| Naziv | Akronim | Številka projekta | Financer |
|---|---|---|---|
| Biotehnologija in sistemska biologija rastlin | P4-0165-2022 |
Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije |
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| Medsebojna povezanost z redoks stanjem posredovane signalizacije pri odpornosti krompirja na viruse | Z4-3217-2021 |
Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije |
Datoteke, ki spadajo k objavi
https://www.jove.com/t/65824/studying-cell-death-initiation-using-a-digital-microscope
Vir: Osebne bibliografije
in: SICRIS
