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  • Live-cell imaging by confocal raman and fluorescence microscopy recognizes the crystal structure of CalciumCarbonate particles in HeLa cells [Elektronski vir]
    Abalymov, Anatolii A. ...
    Porous calcium carbonate (CaCO3) vaterite particles are very attractive templates for the encapsulation of pharmaceuticals and for the construction of hollow polyelectrolyte capsules, sensors, and ... enzyme-catalyzed reactors. Although CaCO3 is biocompatible and biodegradable, little is known about the intercellular behavior and properties of vaterite particles in the cytoplasm of cells. In this work, the authors combine confocal Raman and fluorescent microscopy for the imaging of porous CaCO3 vaterite particles in HeLa cells to study the uptake and status of the particles inside the cells in real time. Analysis of the fluorescence images shows that the particles penetrated the plasma membrane 3 h after being added to the cell culture and that the internalization of the particles continued up to 48 h. The crystal structure of individual vaterite particles in the cytoplasm of HeLa cells did not obviously change for 144 h. For clusters of particles, however, the authors identify Raman spectroscopic signatures of the stable calcite phase after 72 h of incubation, confirming an ion-exchange mechanism of vaterite transformation to calcite. The results indicate that these imaging approach to examining inorganic particles in living cells may have theranostic applications.
    Vir: Biotechnology journal [Elektronski vir]. - ISSN 1860-7314 (Vol. 13, iss. 11, 2018, str. 1800071(1-8))
    Vrsta gradiva - e-članek ; neleposlovje za odrasle
    Leto - 2018
    Jezik - angleški
    COBISS.SI-ID - 178321923

    Povezava(-e):

    https://onlinelibrary.wiley.com/doi/full/10.1002/biot.201800071
    DOI

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