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  • Postmortem chondrocyte viability in porcine articular cartilage [Elektronski vir] : influence of time, temperature, and burial under winter conditions
    Cvetko, Marko, veterina ...
    The aim of the present study was to investigate the effects of time, temperature, and burial in a natural environment on the viability of chondrocytes in porcine femoral condyles using confocal laser ... scanning microscopy. Hind trotters from 10 pigs were buried or left unburied. Samples were collected daily and stained with a combination of vital dyes (calcein-AM and ethidium homodimer-1). The chondrocytes showed an intense staining corresponding to their vitality. In the first 3 days, viability decreased slowly and showed no statistical difference between buried and unburied samples. After the first 3 days, it decreased rapidly, with the viability of the buried samples being 66% on day 4, decreasing to 25% on day 8 and to 16% on day 10, while in the unburied samples it decreased to 43% on day 4, 13% on day 8 and 5% on day 10. Our results indicate a time, temperature, and burial dependent decrease in chondrocyte viability and suggest the use of chondrocyte viability as a marker for estimating PMI in both the natural environment and in animals, as well as its potential use in humans.
    Vir: Journal of forensic sciences [Elektronski vir]. - ISSN 1556-4029 (Vol. 69, no. 3, 2024, str. 1094-1101)
    Vrsta gradiva - e-članek ; neleposlovje za odrasle
    Leto - 2024
    Jezik - angleški
    COBISS.SI-ID - 189439235

    Povezava(-e):

    https://onlinelibrary.wiley.com/doi/10.1111/1556-4029.15503
    Repozitorij Univerze v Ljubljani – RUL
    DOI

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