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Effects of electroporation on the function of sarco/endoplasmic reticulum ▫$Ca^{2+}$▫-ATPase and ▫$Na^+$▫,▫$K^+$▫-ATPase in H9c2 cells [Elektronski vir]Jan, Vid ; Jusović, Maida ; Miklavčič, DamijanPulsed field ablation (PFA) is a promising new treatment for atrial fibrillation (AF), in which pulmonary vein isolation is achieved by irreversible electroporation. Electroporation causes ATP to ... leak through the permeabilized membrane. ATP is required both for the healing of the cell membrane and for the functioning of ion pumps, such as sarco/endoplasmic reticulum ▫$Ca^{2+}$▫-ATPase (SERCA) or ▫$Na^+$▫,▫$K^+$▫-ATPase (NKA), which play a key role in maintaining continuous contractions of the heart muscle. We investigated the effects of electroporation on the expression of ion pumps and possible correlations with the activation of AMPK, the main energy sensor in cells. H9c2 rat cardiac cells were exposed to either monopolar or bipolar (H-FIRE) pulses. Cells lysed 4 or 24 h after electroporation were used for mRNA and protein expression analyses. Overall, both pulse protocols caused a dosedependent downregulation of crucial SERCA and NKA isoforms, except for NKA▫$\alpha$▫2 and ▫$\beta$▫3, which were upregulated after 24 h. Monopolar pulses also decreased the phosphorylation of FXYD1, which may cause an inhibition of NKA activity. Both pulse protocols caused an increased AMPK activity, which may decrease both SERCA and NKA activity via calcium/calmodulin-dependent protein kinase. Our results provide important new insights into what happens in surviving cardiomyocytes after they are exposed to PFA.Vir: Applied sciences [Elektronski vir]. - ISSN 2076-3417 (Vol. 14, iss. 7 [article no.] 2695, Apr.-1 2024, 21 str.)Vrsta gradiva - e-članek ; neleposlovje za odrasleLeto - 2024Jezik - angleškiCOBISS.SI-ID - 190227203
Avtor
Jan, Vid |
Jusović, Maida |
Miklavčič, Damijan
Teme
ireverzibilna ekektroporacija |
reverzibilna elektroporacija |
ablacija s pulznim električnim poljem |
Ca2+-ATPaza sarko/endoplazemskega retikuluma |
Na+ |
K+-ATPaza |
atrijska fibrilacija |
aktivacija AMPK |
H-FIRE pulzi |
celice H9c2 |
irreversible electroporation |
reversible electroporation |
pulsed field ablation |
sarco/endoplasmic reticulum Ca2+-ATPase |
Na+ |
K+-ATPase |
atrial fibrillation |
AMPK activation |
H-FIRE pulses |
H9c2 cells
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Avtor | Jan, Vid ; Jusović, Maida ; Miklavčič, Damijan |
Naslov | Effects of electroporation on the function of sarco/endoplasmic reticulum ▫$Ca^{2+}$▫-ATPase and ▫$Na^+$▫,▫$K^+$▫-ATPase in H9c2 cells [Elektronski vir] |
Datum objave | 2024-03-22 |
COBISS.SI-ID | 190227203 |
Verzija objave v repozitoriju | Založnikova različica |
Licenca objave v repozitoriju | Creative Commons Priznanje avtorstva 4.0 Mednarodna |
Embargo | Ni določeno |
Projekti, iz katerih je bila financirana objava
Naziv | Akronim | Številka projekta | Financer |
---|---|---|---|
Elektroporacija v biologiji, biotehnologiji in medicini | P2-0249-2022 |
Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije |
|
Mreža raziskovalnih infrastrukturnih centrov Univerze v Ljubljani (MRIC UL) | I0-0022-2022 |
Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije |
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Jan, Vid | 38241 |
Jusović, Maida | 57297 |
Miklavčič, Damijan | 10268 |
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