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  • Evaluation of a fibrin-based skin substitute prepared in a defined keratinocyte medium
    Krašna, Metka ...
    The purpose of this study was to evaluate the influence of fibrin glue and aprotinin on the growth of adult human skin keratinocytes in defined serum-free conditions. The keratinocytes were cultured ... on cell culture plastics and on a fibrin matrix prepared from fibrin glue. The cell growth wasmeasured by MTT assay, while the growth of clonogenic keratinocytes was evaluated by colony assay and expressed as colony-forming efficiency (CFE). The clonogenic potential of keratinocytes released from subconfluent and confluent cultures grown on fibrin glue was also studied by the colony assay. In comparison to a plastic culture surface the fibrin glue had significantly (P < 0.05) increased the clonogenic potential of keratinocytes, as well as enhanced their growth. Keratinocytes released from subconfluent cultures grownon fibrin glue attained a significantly (P< 0.05) higher percentage of clonogenic cells than their confluent parallels. At 75, 150, 300 and 450 KIU/ml aprotinin did not influence the growth of keratinocytes (P > 0.2). A fibrin-based skin substitute produced in the defined keratinocyte medium couldbe safely used to treat a number of skin defects.
    Vir: International journal of pharmaceutics. - ISSN 0378-5173 (Letn. 291, 2005, str. 31-37)
    Vrsta gradiva - članek, sestavni del
    Leto - 2005
    Jezik - angleški
    COBISS.SI-ID - 19192025

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