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  • The influence of Lidocaine on cell membrane permeabilization and cell survival after electroporation [Elektronski vir]
    Blažič, Anja, biologinja in mikrobiologinja ...
    Electroporation is a phenomenon in which exposure of a cell to pulsed electric field causes the formation of pores in the cell membrane. During the period of increased membrane permeability, ... otherwise impermeable molecules can be introduced into the cell or extracted from the cell. If the cell survives, this is called reversible electroporation; if the cell dies, this is considered irreversible electroporation. Both reversible and irreversible electroporation are used in different applications in medicine, including treatment of solid tumors. In these treatments, Lidocaine is often used as a local anesthetic to mitigate pain. However, a few studies also suggested that Lidocaine acts as a cell sensitizer for reversible and/or irreversible electroporation. To further study the potential of Lidocaine as a sensitizing agent, our investigation focused on assessing Lidocaine's effect on cell membrane permeabilization and cell survival in in vitro experiments, whereby 4 different cells lines (B16F1, C2C12, CHO and NS-HEK) were exposed to 8 x 100 µs pulses at repetition frequency of 1 Hz, as conventionally used for electrochemotherapy treatment. Our results suggest that 10 mM Lidocaine, dissolved in a physiological electroporation solution, has a minor effect on cell membrane permeabilization. However, a significant decrease in cell survival is observed, which can be attributed mainly to Lidocaine's inherent cytotoxicity.
    Vir: ATRASC 2024 [Elektronski vir] : 4th URSI Atlantic Radio Science Meeting : Gran Canaria, 19-24 May 2024 ([4] str.)
    Vrsta gradiva - prispevek na konferenci ; neleposlovje za odrasle
    Leto - 2024
    Jezik - angleški
    COBISS.SI-ID - 198161411

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