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cDNA cloning, tissue distribution, and identification of the catalytic triad of monoglyceride lipase. Evolutionary relationship to esterases, lysophospholipases, and haloperoxidasesKarlsson, Marie ...Monoglyceride lipase catalyzes the last step in the hydrolysis of stored triglycerides in the adipocyte and presumably also complements the action of lipoprotein lipase in degrading triglycerides ... from chylomicrons and very low density lipoproteins. Monoglyceride lipase was cloned from a mouse adipocyte cDNA library. The predicted amino acid sequence consisted of 302 amino acids, corresponding to a molecular weight of 33,218. The sequence showed no extensive homology to other known mammalian proteins, but a number of microbial proteins, including two bacterial lysophospholipases and a family ofhaloperoxidases, were found to be distantly related to this enzyme. By meansof multiple sequence alignment and secondary structure prediction, the structural elements in monoglyceride lipase, as well as the putative catalytictriad, were identified. The residues of the proposed triad, Ser-122, in a GXSXG motif, Asp-239, and His-269, were confirmed by site-directed mutagenesis experiments. Northern blot analysis revealed that monoglyceride lipase is ubiquitously expressed among tissues, with a transcript size of about 4 kilobases.Vir: The Journal of biological chemistry. - ISSN 0021-9258 (Letn. 272, št. 43, 1997, str. 27218-27223)Vrsta gradiva - članek, sestavni delLeto - 1997Jezik - angleškiCOBISS.SI-ID - 21679321
Avtor
Karlsson, Marie |
Contreras, Juan Antonio |
Hellman, Ulf |
Tornquist, Hans |
Holm, Cecilia
Teme
Evolution, Molecular |
Adipocytes |
Enzymology |
Amino Acid Sequence |
Animals |
Base Sequence |
Cos Cells |
Catalysis |
Cloning, Molecular |
Dna, Complementary |
Esterases |
Chemistry |
Lysophospholipase |
Chemistry |
Mice |
Molecular Sequence Data |
Monoacylglycerol Lipases |
Biosynthesis |
Chemistry |
Genetics |
Organ Specificity |
Peroxidases |
Chemistry |
Rats |
Recombinant Proteins |
Biosynthesis |
Chemistry |
Sequence Alignment |
Sequence Homology, Amino Acid |
Transfection |
Evolucija molekularna |
Adipociti |
Aminokislinsko zaporedje |
Baze, zaporedje |
COS celice |
DNA komplementarna |
Esteraze |
Kataliza |
Kloniranje molekulsko |
Lizofosfolipaza |
Miši |
Molekulsko zaporedje, podatki |
Monoacilglicerolne lipaze |
Organ, specifičnost |
Peroksidaze |
Podgane |
Rekombinantne beljakovine |
Transfekcija |
Zaporedje razvrstitev |
Zaporedna homolognost aminokislin |
Živali
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Karlsson, Marie | ![]() |
Contreras, Juan Antonio | 28452 |
Hellman, Ulf | ![]() |
Tornquist, Hans | ![]() |
Holm, Cecilia | ![]() |
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