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Crystal structure of brefeldin A esterase, a bacterial homolog of the mammalian hormone-sensitive lipaseWei, Yunyi ...Brefeldin A esterase (BFAE), a detoxifying enzyme isolated from Bacillus subtilis, hydrolyzes and inactivates BFA, a potent fungal inhibitor of intracellular vesicle-dependent secretory transport and ... poliovirus RNA replication. We have solved the crystal structure of BFAE and we discovered that the previously reported amino acid sequence was in serious error due to frame shifts in the cDNA sequence. The correct sequence, inferred from the experimentally phased electron density map, revealed that BFAE is a homolog ofthe mammalian hormone sensitive lipase (HSL). It is a canonical alpha/beta hydrolase with two insertions forming the substrate binding pocket. The enzymecontains a lipase-like catalytic triad, Ser 202, Asp 308 and His 338, consistent with mutational studies that implicate the homologous Ser 424, Asp 693 and His 723 in the catalytic triad in human HSL.Vir: Nature structural biology. - ISSN 1072-8368 (Letn. 6, št. 4, 1999, str. 340-345)Vrsta gradiva - članek, sestavni delLeto - 1999Jezik - angleškiCOBISS.SI-ID - 21680089
Avtor
Wei, Yunyi |
Contreras, Juan Antonio |
Sheffield, Peter |
Osterlund, Torben |
Derewenda, Urszula |
Kneusel, R.E. |
Matern, Ulrich |
Holm, Cecilia |
Derewenda, Zygmund S.
Teme
Amino Acid Sequence |
Animals |
Bacillus Subtilis |
Chemistry |
Enzymology |
Bacterial Proteins |
Chemistry |
Genetics |
Metabolism |
Base Sequence |
Carboxylic Ester Hydrolases |
Chemistry |
Genetics |
Metabolism |
Cholesterol Esterase |
Drug Effects |
Metabolism |
Crystallography, X-Ray |
Hormones |
Pharmacology |
Mammals |
Models, Molecular |
Molecular Sequence Data |
Protein Conformation |
Protein Folding |
Reproducibility Of Results |
Sequence Analysis |
Methods |
Sequence Homology, Amino Acid |
Aminokislinsko zaporedje |
Bacillus subtilis |
Bakterijske beljakovine |
Baze, zaporedje |
Beljakovine, gubanje |
Beljakovinska konformacija |
Holesterolna esteraza |
Hormoni |
Karboksilni ester, hidrolaze |
Kristalografija radiografska |
Modeli molekularni |
Molekulsko zaporedje, podatki |
Ponovljivost rezultatov |
Sesalci |
Zaporedje, analiza |
Zaporedna homolognost aminokislin |
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Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
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Wei, Yunyi | ![]() |
Contreras, Juan Antonio | 28452 |
Sheffield, Peter | ![]() |
Osterlund, Torben | ![]() |
Derewenda, Urszula | ![]() |
Kneusel, R.E. | ![]() |
Matern, Ulrich | ![]() |
Holm, Cecilia | ![]() |
Derewenda, Zygmund S. | ![]() |
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