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  • Priprava dendritskih celic iz periferne krvi : magistrska naloga
    Hajdinjak, Tine
    Introduction In cancer immunotherapy and other fields of immune system modulation, dendritic cells (DC) are gaining more and more importance. They bridge innate and specific immunity by influencing ... NK cells. They are the onlyantigen-presenting cells capable of initiating a new T cell response, but they can also induce tolerance. Purpose We adopted and modified different methods for DC production. We studied the possibility of correlating the actual form of DC to their phenotype. DC development in vivo is subdivided in many stages, while in vitro only immature and mature forms are acknowledged. We supposed that it shoud also be possible to identify different immature stages of DC in vitro. In one-way allogeneic MLR, a basic functional test, thestimulating capacity of DCs is typically evaluated on day 5 or 6 of the cell culture. We anticipated that due to the stimulating properties of DCs, itwould be possible to reveal significant proliferative responses earlier thanthat, thus reducing the performing time for the test. Materials and methods We prepared DCs in cell cultures using mononuclear cells, isolated from buffy coats from healthy blood donors. Monocytes were enriched with the use of Dynal immunomagnetic beads. We used calcium ionophores and cytokines IL-4, GM- CSF and TNFalpha. Using light microscopy and phase-contrast, morphological features of monocyte to DC transformation were followed. The expression of leukocyte antigens and the ability of phagocytosis were assessedwith the use of flow cytometry. Transmission electron microscopy and classic immunofluorescence microphotographs of DCs were also taken. In one-wayallogeneic MLR, the kinetics of proliferative response was followed with the the use of a liquid (beta-scintillation counter to measure the DNA incorporation of 3H- thymidine. Results The cell culture medium with cytokinesshould contain IL-4 at 800Užml and GM-CSF at 800Užml in a 6-day culture. (Abstract truncated at 2000 characters).
    Vrsta gradiva - magistrsko delo
    Založništvo in izdelava - Ljubljana : [T. Hajdinjak], 2000
    Jezik - slovenski
    COBISS.SI-ID - 2175508

Knjižnica/institucija Kraj Akronim Za izposojo Druga zaloga
MF, Centralna medicinska knjižnica, Ljubljana Ljubljana CMK na dom 1 izv.
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