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Calcium dynamics and compartmentalization in leech neuronsAndjelić, SofijaThe aim of this proj ect was to study how and where action potentials arise and propagate in the arborizations of identified neurons in the central nervous system of the leech. A major aim was to ... assess whether the entry of calcium is localized to distinct regions of the cells and to determine whetherthere are significant differences in calcium channel distribution between different types of neurons. A combination of electrophysiological techniques, optical recording and image analysis was used to approach these problems. I developed an experimental set-up for optical recordings of calciumtransients b a fast CCD-camera. By use of calcium sensitive dyes I analysed in detail optical responses to electrical stimulation of neurons and the density of calcium channels, spatially and temporarily, in different neural cell types, including mechanosensory neurons and motoneurons. Fluorescence changes (deltaF/F) of the membrane impermeable calcium indicator Oregon Green were measured. The dye was pressure injected into the soma of neurons under investigation. DeltaF/F caused by a single action potential (AP)in mechanosensory neurons had approximately the same amplitude and time course in the soma and in distal processes. By contrast, in other neurons suchas the Anterior Pagoda neuron, the Annulus Erector motoneuron, the L motoneuron and other motoneurons, APs evoked by passing depolarizing current in the soma produced much larger fluorescence changes in distal processes thanin the soma. When APs were evoked by stimulating one distal axon through the root, deltaF/F was large in all distal processes, but very small in the soma. These results confirm and extend previous electrophysiological data which demonstrate that the soma of a motoneuron in the leech, as in many otherinvertebrates, does not generate action potentials (Stuart, 1970; Muller and Nicholls, 1974; Goodman and Heiltler, 1979). (Abstract truncated at 2000 characters)Vrsta gradiva - disertacijaZaložništvo in izdelava - Trieste : [S.Andjelić], 2006Jezik - angleškiCOBISS.SI-ID - 21775833
Avtor
Andjelić, Sofija
Drugi avtorji
Torre, Vincent, 1950-
Teme
Leeches |
Neurons |
Calcium Channels |
Motor Neurons |
Mechanoreceptors |
Action Potentials |
Calcium |
Electric Stimulation |
Fluorescent Dyes |
Kalcijevi kanalčki |
Mehanoreceptorji |
Motorični nevroni |
Nevroni |
Pijavke |
Akcijski potenciali |
Električna stimulacija |
Fluorescentna barvila |
Kalcij
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