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  • Analysis of the real EADGENE data set : comparison of methods and guidelines for data normalisation and selection of differentially expressed genes
    Jaffrézic, Florence ...
    A large variety of methods has been proposed in the literature for microarray data analysis. The aim of this paper was to present technoques used by the EADGENE (European Animal Disease Genomics ... Network of Excellence) WP1.4 participants for data quality control, normalisation and statistical methods for the detection of differentially expressed genes in order to provide some more general data analysis guidleines. All the workshop participants were given a real data set obtained in an EADGENE founded micrroarray study lookingat the gene expression changes following artificial infection with two different mastitis causing bacteria: Escherichia coli and Staphylococcus aureus. It was ressuring to see that most of the teams found the same main biological results. In fact, most of the differentially expressed genes were found for infection by E. coli between uninfected and 24 h challenged udder quarters. Very little transcriptional variation was observed for the bacteria S. aureus. Lists of differentially expressed genes found by the different research teams were, however, quite dependent on the method used, especially concerning the data quality control step. These analyses also emphasised a biological problem of cross-talk between infected and uninfected quarters which will have to be dealt with for further microarray studies.
    Vir: Genetics selection evolution. - ISSN 0999-193X (Letn. 39, št. 6, 2007, str. 633-650)
    Vrsta gradiva - članek, sestavni del
    Leto - 2007
    Jezik - angleški
    COBISS.SI-ID - 2220424

vir: Genetics selection evolution. - ISSN 0999-193X (Letn. 39, št. 6, 2007, str. 633-650)
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