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  • Determination of derivatized amino acids in human embryo culture media by gas chromatography
    Križman, Mitja ; Virant-Klun, Irma ; Prošek, Mirko
    An adequate analytical method for determination of amino acids can provide a better insight in the metabolism of in vitro human embryo cultures, increasingthe success rate of embryo implantation. ... Since individual amino acidamounts per embryo occur in the nanogram range, GC was the technique of choice, due to its inherent sensitivity and high sample throughput. Amino acids were analyzed as alkyl formate derivatives. The limits of detection (LOD) of all amino acids involved were in the sub-nmol range. The high risk ofsample contamination proved to be the major analytical issue, but it could be overcome. For an extended method sensitivity, a simple preconcentration step could also be used.
    Vir: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. - ISSN 1570-0232 (Vol. B 858, iss. 1/2, 2007, str. 292-295)
    Vrsta gradiva - članek, sestavni del
    Leto - 2007
    Jezik - angleški
    COBISS.SI-ID - 23209433

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