Akademska digitalna zbirka SLovenije - logo
VSE knjižnice (vzajemna bibliografsko-kataložna baza podatkov COBIB.SI)
  • Detection of the status of tumour-infiltrating CD4+ T-cell subpopulations in squqmous cell carcinoma of the head and neck (HNSCC) using real-time RT-PCR detection of CD4 mRNA
    Smerkolj, Sava ; Volavšek, Metka ; Glavač, Damjan
    Several studies have reported tumour infiltrating CD4+ T cells as a favourableprognostic factor in some types of cancer. We investigated 37 head and neck squamous cell carcinomas (HNSCC) at different ... stages, using imnmnohistochemical staining for CD4+ infiltrates and real-time reverse transcription polymerase chain reaction (RT-PCR) detection of CD4 mRNA. The CD4+ infiltrates were evaluated and expressed as a percentage according to theratio of CD4+ T cells to epithelial cells in the cancer cell nests and to the overall inflammatory cell infiltrate in the tumor stroma. The CD4 mRNA expression level strongly correlated with the CD4+ infiltration score in the cancer epithelium (rs = 0.858, P < 0.001) and in the cancer stroma (rs = 0.797, P < 0.001). These results indicate that the real-time RT-PCR assay is asensitive and reliable method for the detection of CD4 mRNA, and that it could he used to reassess CD4+ infiltration status in resected specimens from patients with HNSCC.
    Vir: Cellular & molecular biology letters : an international journal : cellular membranes, cytoskeleton, nuclear architecture, gene expression, biomolecular interactions, biotechnology. - ISSN 1425-8153 (Letn. 12, 2007, str. 308-316)
    Vrsta gradiva - članek, sestavni del
    Leto - 2007
    Jezik - angleški
    COBISS.SI-ID - 23430617

Vnos na polico