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  • Semi-quantitative method for detection of fetomaternal hemorrhage with gel agglutination technique
    Bizjak, Bojana, 1961-
    Background: in order to prevent maternal sensibilization with the child's RhD positive red blood cells (RBC), RhD negative mothers are treated with RhD-immune globulin (RhD Ig). The standard dosage ... in Slovenia is 300 [micro]g of RhD Ig in the 28th week of gestation and 150 [micro]g of RhD Ig after delivery. 150 [micro]g of RhD Ig suffers for a 7,5 ml of fetal RhD positive RBCs, representing an approximately 0,25 % of fetal RBC subpopulation in a maternal circulation. Adequate administration of RhD Ig requires an accurate measurement of the volume of RhD positive RBC in the circulation of a mother. Therefore, we looked for a simple and rapid screening test for detection of more than 0,25 % of fetal RBC subpopulation. Methods: A mother's postpartal sample is incubated with a monoclonal anti-D serum from ID-FMH Screening Test (DiaMed, Switzerland). Commercial adult RhD-negative RBC with various amounts of fetal RhD positive RBC (0.1 %, 0.2 % and 0.4 %) of the same manufacturer are tested in parallel, to aid interpretation. After incubation, the supernatants are tested against R2R2 RBCs in order to determine the reduction of anti-D reactivity. We compared ID-FMH Screening Test results with the Kleinhauer-Betke test, which is based on fetal hemoglobin's resistance to acidelution, unlike adult hemoglobin. A commercial Sure-tech Diagnostic kit was used. Results: 386 postpartum samples from RhD-negative women who delivered RhD-positive infants were tested using both tests. In four samples fetomaternal hemorrhage (FMH) exceeded 7.5 ml of fetal RBCs (15 ml of whole blood) and mothers needed more than 150 [micro]g of RhD Ig. In these cases both employed tests showed more than 0.4 % of fetal RBC. Conclusion: The gel agglutination technique is a rapid, simple, semi-quantitative method for routine screening for clinically significant FMH.
    Vir: 10 let uporabe gelske aglutinacijske metode pri transfuzijskih preiskavah v Sloveniji = 10 years of using gel agglutination method for transfusion testing in Slovenia : zbornik predavanj : = book of the lectures (Str. 48-52)
    Vrsta gradiva - prispevek na konferenci ; neleposlovje za odrasle
    Leto - 2006
    Jezik - angleški
    COBISS.SI-ID - 2355007

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