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  • Evolution of Nova-dependent splicing regulation in the brain
    Jelen, Nejc ...
    A large number of alternative exons are spliced with tissue-specific patterns,but little is known about how such patterns have evolved. Here, we study the conservation of the neuron-specific splicing ... factors Nova1 and Nova2and of the alternatively spliced exons they regulate in mouse brain. Whereas Nova RNA binding domains are 94% identical across vertebrate species, Nova-dependent splicing silencer and enhancer elements (YCAY clusters) show much greater divergence, as less than 50% of mouse YCAY clusters are conservedat orthologous positions in the zebrafish genome. To study the relation between the evolution of tissue-specific splicing and YCAY clusters, we compared the brain-specific splicing of Nova-regulated exons in zebrafish, chicken, and mouse. The presence of YCAY clusters in lower vertebrates invariably predicted conservation of brain-specific splicing across species, whereas their absence in lower vertebrates correlated with a loss of alternative splicing. We hypothesize that evolution of Nova-regulated splicingin higher vertebrates proceeds mainly through changes in cis-acting elements, that tissue-specific splicing might in some cases evolve in a singlestep corresponding to evolution of a YCAY cluster, and that the conservation level of YCAY clusters relates to the functions encoded by the regulated RNAs.
    Vir: PLOS genetics. - ISSN 1553-7390 (Letn. 3, št. 10, 2007, str. 1838-1847)
    Vrsta gradiva - članek, sestavni del
    Leto - 2007
    Jezik - angleški
    COBISS.SI-ID - 23743705

vir: PLOS genetics. - ISSN 1553-7390 (Letn. 3, št. 10, 2007, str. 1838-1847)
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