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  • Adhesion molecules involved in the binding and subsequent engulfment of thymocytes by a rat thymic epithelial cell line
    Čolić, Miodrag ...
    A rat thymic epithelial cell (TEC) line (R-TNC.1) was established from a long-term TEC culture. Based on its ultrastructure, phenotype and cytokeratin profile, this line was characterized as a type ... of cortical TEC. R-TNC.1 cells had nursing activity which was manifested by the binding and subsequent engulfment of thymocytes. The role of adhesion molecules involved in these processes was studied extensively using a coculture of resting thymocytes and unstimulated or interferon-gamma (IFN-gamma)-stimulated R-TNC.1 cells. It was found that a number of adhesion molecules, such as CD2, CD4, CD8, LFA-1, CD18,ICAM-1 and Thy-1, was partly involved in the nursing activity. The effect of monoclonal antibodies (mAb) to these molecules depended on the incubation time and stimulation of R-TNC.1 cells. The inhibitory effect of mAbto CD2, LFA-1, CD18 and ICAM-1 on thymocyte engulfment was higher than their effect on thymocyte binding to the R-TNC.1 line. In addition, a LFA-1/CD18-dependent/ICAM-1-independent adhesion pathway was identified when unstimulated R-TNC.1 cells with minimal expression of ICAM-1 were used. The combination of inhibitory mAb did not completely abrogate the nursing activityof the R-TNC.1 line, suggesting the possible involvement of some other adhesion molecules.
    Vir: Immunology. - ISSN 0019-2805 (Letn. 83, št. 3, 1994, str. 449-456)
    Vrsta gradiva - članek, sestavni del
    Leto - 1994
    Jezik - angleški
    COBISS.SI-ID - 23943129

vir: Immunology. - ISSN 0019-2805 (Letn. 83, št. 3, 1994, str. 449-456)
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