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  • Tkivno-specifično uravnavanje izražanja holesterogenih genov : magistrsko delo = Tissue-specific regulation of cholesterogenic genes expression : master of science thesis
    Fon Tacer, Klementina
    Lanosterol 14alpha-demethylase is the only member of the cytochrome P450 gene superfamily involved in the housekeeping pathway of cholesterol biosynthesis. CYP51 converts lanosterol to FF-MAS, ... meiosis activating sterol, isolated from human follicular fluid. FFMAS and its reduced form T-MAS (meiosis activating sterol, isolated from bull testis) are postsqualene cholesterol intermediates,accumulating in mammalian gonads. FF-MAS and T-MAS are able to trigger reinitiation of oocyte meiosis in in vitro assay. In mammals, CYPS 1 is expressed in all tissues with the highest level in testis, precisely in haploid male germ cells, due to the appearance of shorter testis-specific transcripts. Cholesterol biosynthesis is precisely regulated through cholesterol negative feedback loop by transcription factors SREBP (sterol responsive element binding protein). Expression of CYP51 gene is regulated through sterolžSREBP dependent mechanism as well as through mechanism dependent on cAMPžCREMtau. In haploid male germ cells, cholesterol-independentcAMPžCREMtau dependent mechanism predominates. We investigated the expression of cholesterogenic genes in mouse and rat testis. In rat testis, genes encoding enzymes involved in MAS synthesis (HMG CoA-synthase, HMG CoA-reductase, FPP synthase, squalene synthase and CYP51) are expressed at higher level compared to genes encoding enzymes responsible for MAS conversion toward cholesterol (sterol C4-methyl oxidase, sterol delta 7-reductase). Expression of pre-MAS genes in testis is upregulated during sexual development of the rat. Expression level of post-MAS genes in rat testis is low and even decreases during maturation of the rat. Expression of cholesterogenic genes in testis is, in contrast to liver, discordant. (Abstract truncated at 2000 characters).
    Vrsta gradiva - magistrsko delo
    Založništvo in izdelava - Ljubljana : [K. Fon Tacer], 2002
    Jezik - slovenski
    COBISS.SI-ID - 2399508

Knjižnica/institucija Kraj Akronim Za izposojo Druga zaloga
MF, Centralna medicinska knjižnica, Ljubljana Ljubljana CMK na dom 1 izv.
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