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  • Complete nucleotide sequence of Tn1721: gene organization and a novel gene product with features of a chemotaxis protein
    Allmeier, H... ...
    The complete 11,139-nucleotide sequence of transposon Tn1721 has been determined. It contains three 38-bp inverted repeats, and (in this order) a new orfI, a resolution site (res), genes encoding ... resolvase (tnpR), transposase (tnpA), tetracycline-resistance (TcR) repressor (tetR), TcR (tetA) and a truncated transposase gene (tnpA'). The modulator origin of Tn1721 from at least three separate sources is supported by the distinctive codon usages of orfI, tnpR/tnpA and tetR/tetA, and by sequence similarities with Tn501 (tnpR/tnpA) and RP1 (tetR/tetA). The ORFI-encoded 56-kDa polypeptide exhibits features of a methyl-accepting chemotaxis protein (MCP) with a conserved signal domain and a potential transmembrane domain; this polypeptide cross-reacts with anti-MCP antiserum. Like chemotaxis genes, orfI is transcribed from a sigma 28-like promoter. The overexpressed orfI gene product interferes with MCP-dependent chemotaxis suggesting that it completes for soluble transducer protein(s) in the cell. The potential selective advantage of this novel transposon-borne gene is discussed.
    Vir: Gene. - ISSN 0378-1119 (Letn. 111, št. 1, 1992, str. 11-20)
    Vrsta gradiva - članek, sestavni del
    Leto - 1992
    Jezik - angleški
    COBISS.SI-ID - 2591961

vir: Gene. - ISSN 0378-1119 (Letn. 111, št. 1, 1992, str. 11-20)
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