-
Amyotrophic lateral sclerosis immunoglobulins G enhance the mobility of Lysotracker-labelled vesicles in cultured rat astrocytesStenovec, Matjaž ...Aim: We examined the effect of purified immunoglobulins G (IgG) from patients with amyotrophic lateral sclerosis (ALS) on the mobility and exocytotic release from Lysotracker-stained vesicles in ... cultured rat astrocytes. Methods:Time-lapse confocal images were acquired, and vesicle mobility was analysed before and after the application of ALS IgG. The vesicle counts were obtained to assess cargo exocytosis from stained organelles. Results: At rest,when mobility was monitored for 2 min in bath with Ca(2+) , two vesicle populations were discovered: (1) non-mobile vesicles (6.1%) with total track length (TL) < 1 mum, averaging at 0.33 +/- 0.01 mum (n = 1305) and (2) mobile vesicles (93.9%) with TL > 1 mum, averaging at 3.03 +/- 0.01 mum (n = 20 200).ALS IgG (0.1 mg mL(-1) ) from 12 of 13 patients increased the TL of mobile vesicles by approx. 24% and maximal displacement (MD) by approx. 26% within 4 min, while the IgG from control group did not alter the vesicle mobility. The mobility enhancement by ALS IgG was reduced in extracellular solution devoid of Ca(2+) , indicating that ALS IgG vesicle mobility enhancement involves changes in Ca(2+) homeostasis. To examine whether enhanced mobility relates to elevated Ca(2+) activity, cells were stimulated by 1 mm ATP, a cytosolic Ca(2+) increasing agent, in the presence (2 mm) and in the absence of extracellular Ca(2+) . ATP stimulation triggered an increasein TL by approx. 7% and 12% and a decrease in MD by approx. 11% and 1%, within 4 min respectively. Interestingly, none of the stimuli triggered the release of vesicle cargo. Conclusion: Amyotrophic lateral sclerosis-IgG-enhanced vesicle mobility in astrocytes engages changes in calcium homeostasis.Vir: Acta physiologica. - ISSN 1748-1708 (Vol. 203, issue 4, 2011, str. 457-471)Vrsta gradiva - članek, sestavni delLeto - 2011Jezik - angleškiCOBISS.SI-ID - 28725465
Avtor
Stenovec, Matjaž |
Milošević, M. |
Petrušić, V. |
Potokar, Maja, 1973- |
Stević, Zorica |
Lobe Prebil, Mateja |
Kreft, Marko |
Trkov, Saša |
Andjus, Pavle |
Zorec, Robert, 1958-
Teme
Amyotrophic Lateral Sclerosis |
Igg |
Astrocytes |
Endosomes |
Cells, Cultured |
Exocytosis |
Lysosomes |
Microscopy, Confocal |
Rats |
Amiotrofična, lateralna skleroza |
IgG |
Astrociti |
Endosomi |
Celične kulture |
Eksocitoza |
Lizosomi |
Mikroskopija konfokalna |
Podgane
![loading ... loading ...](themes/default/img/ajax-loading.gif)
Vnos na polico
Trajna povezava
- URL:
Faktor vpliva
Dostop do baze podatkov JCR je dovoljen samo uporabnikom iz Slovenije. Vaš trenutni IP-naslov ni na seznamu dovoljenih za dostop, zato je potrebna avtentikacija z ustreznim računom AAI.
Leto | Faktor vpliva | Izdaja | Kategorija | Razvrstitev | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Baze podatkov, v katerih je revija indeksirana
Ime baze podatkov | Področje | Leto |
---|
Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
---|---|
Stenovec, Matjaž | 15467 |
Milošević, M. | ![]() |
Petrušić, V. | ![]() |
Potokar, Maja, 1973- | 21390 |
Stević, Zorica | ![]() |
Lobe Prebil, Mateja | 28325 |
Kreft, Marko | 15666 |
Trkov, Saša | 31572 |
Andjus, Pavle | ![]() |
Zorec, Robert, 1958- | 03702 |
Izberite prevzemno mesto:
Prevzem gradiva po pošti
Obvestilo
Gesla v Splošnem geslovniku COBISS
Izbira mesta prevzema
Mesto prevzema | Status gradiva | Rezervacija |
---|
Prosimo, počakajte trenutek.