Akademska digitalna zbirka SLovenije - logo
VSE knjižnice (vzajemna bibliografsko-kataložna baza podatkov COBIB.SI)
PDF
  • STING-dependent interferon-[lambda]1 induction in HT29 cells, a human colorectal cancer cell line, after gamma-radiation
    Chen, Jianzhou ...
    Purpose To investigate the induction of type III interferons (IFNs) in human cancer cells by gamma-rays. Methods and Materials Type III IFN expression in human cancer cell lines after gamma-ray ... irradiation in vitro was assessed by reverse transcription%quantitative polymerase chain reaction and enzyme-linked immunosorbent assay. Signaling pathways mediating type III IFN induction were examined by a variety of means, including immunoblotting, flow cytometry, confocal imaging, and reverse transcription%quantitative polymerase chain reaction. Key mediators in these pathways were further explored and validated using gene CRISPR knockout or short hairpin RNA knockdown. Results Exposure to gamma-rays directly induced type III IFNs (mainly IFNL1) in human cancer cell lines in dose- and time-dependent fashions. The induction of IFNL1 was primarily mediated by the cytosolic DNA sensors%STING%TBK1%IRF1 signaling axis, with a lesser contribution from the nuclear factor kappa b signaling in HT29 cells. In addition, type III IFN signaling through its receptors serves as a positive feedback loop, further enhancing IFN expression via up-regulation of the kinases in the STING%TBK1 signaling axis. Conclusions Our results suggest that IFNL1 can be up-regulated in human cancer cell lines after gamma-ray treatment. In HT29 cells this induction occurs via the STING pathway, adding another layer of complexity to the understanding of radiation-induced antitumor immunity, and may provide novel insights into IFN-based cancer treatment.
    Vir: International journal of radiation oncology, biology, physics. - ISSN 0360-3016 (Vol. 101, no. 1, 2018, str. 97-106)
    Vrsta gradiva - članek, sestavni del
    Leto - 2018
    Jezik - angleški
    COBISS.SI-ID - 2899323
    DOI

Vnos na polico